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T. G. Floyd, J. Song, A. Hapeshi, S. Laroque, M. Hartlieb, S. Perrier
Bottlebrush Copolymers for Gene Delivery: Influence of Architecture, Charge Density, and Backbone Length on Transfection Efficiency
J. Mater. Chem. B 2022, 10, 3696-3704
DOI: 10.1039/D2TB00490A
The influence of polymer architecture of polycations on their ability to transfect mammalian cells is probed. Polymer bottle brushes with grafts made from partially hydrolysed poly(2-ethyl-2-oxazoline) are used while varying the length of the polymer backbone as well as the degree of hydrolysis (cationic charge content). Polyplex formation is investigated via gel electrophoresis, dye-displacement and dynamic light scattering. Bottle brushes show a superior ability to complex pDNA when compared to linear copolymers. Also, nucleic acid release was found to be improved by a graft architecture. Polyplexes based on bottle brush copolymers showed an elongated shape in transmission electron microscopy images. The cytotoxicity against mammalian cells is drastically reduced when a graft architecture is used instead of linear copolymers. Moreover, the best-performing bottle brush copolymer showed a transfection ability comparable with that of linear poly(ethylenimine), the gold standard of polymeric transfection agents, which is used as positive control. In combination with their markedly lowered cytotoxicity, cationic bottle brush copolymers are therefore shown to be a highly promising class of gene delivery vectors.
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Welcome to the WEB-SITE of Chemistry at the University of Potsdam. We invite you to get acquainted with our research and teaching expertise, the outstanding equipment of our laboratories and already existing national and international collaborations. You find us in Wissenschaftspark Golm. If you would like to study Chemistry at the University of Potsdam or if you want to benefit from our research activities and collaborate with us, you are cordially welcome.
 
Prof. Dr. Helmut Schlaad
 
 

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Prof. Heiko Möller - Analytical Chemistry

Inorganic Chemistry

Dr. Claudia Pacholski - Inorganic Chemistry

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Prof. André Laschewsky - IAP

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Prof. Yan Lu - HZB

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apl. Prof. Judith Schicks - Multiphase Systems

apl. Prof. Michael U. Kumke - Optical Sensor Technology and Spectroscopy

apl. Prof. Tillmann Klamroth - Computational Chemistry

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PD Dr. Jolanda Hermanns - Didactics of Chemistry

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