 | S. M. J. Khadem, C. Hille, H.-G. Löhmannsröben, I. M. Sokolov
What information is contained in the fluorescence correlation spectroscopy curves, and where
Physical Review E 2016, 94, 022407
DOI: 10.1103/PhysRevE.94.022407 | | We discuss the application of fluorescence correlation spectroscopy (FCS) for characterization of anomalous
diffusion of tracer particles in crowded environments. While the fact of anomaly may be detected by the standard
fitting procedure, the value of the exponent α of anomalous diffusion may be not reproduced correctly for
non-Gaussian anomalous diffusion processes. The important information is however contained in the asymptotic
behavior of the fluorescence autocorrelation function at long and at short times. Thus, analysis of the short-time
behavior gives reliable values of α and of lower moments of the distribution of particles' displacement, which
allows us to confirm or reject its Gaussian nature. The method proposed was tested on the FCS data obtained in
artificial crowded fluids and in living cells. | | Zurück zur Übersicht » |
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